LOCUS       40924_43153       14164 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Targeting vector pTgKCNQ2 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14164)
  AUTHORS   Tomonoh Y, Deshimaru M, Araki K, Miyazaki Y, Arasaki T, Tanaka Y, 
            Kitamura H, Mori F, Wakabayashi K, Yamashita S, Saito R, Itoh M, 
            Uchida T, Yamada J, Migita K, Ueno S, Kitaura H, Kakita A, Lossin C,
            Takano Y, Hirose S.
  TITLE     The kick-in system: a novel rapid knock-in strategy
  JOURNAL   PLoS ONE 9 (2), E88549 (2014)
  PUBMED    24586341
REFERENCE   2  (bases 1 to 14164)
  AUTHORS   Deshimaru M, Miyazaki Y, Arasaki T, Araki K, Hirose S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2009) Contact:Masanobu Deshimaru Fukuoka 
            University, Department of Chemistry, Faculty of Science; Nanakuma 
            8-19-1, Jonan-ku, Fukuoka, Fukuoka 814-0180, Japan
REFERENCE   3  (bases 1 to 14164)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14164)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2014"; volume: "9"; issue: "2"; pages: "E88549"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-NOV-2009) Contact:Masanobu Deshimaru Fukuoka University, 
            Department of Chemistry, Faculty of Science; Nanakuma 8-19-1, 
            Jonan-ku, Fukuoka, Fukuoka 814-0180, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             961..1533
                     /label=DTA
                     /note="diphtheria toxin fragment A"
     intron          1610..1675
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1888..1943
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    2316..7483
                     /gene="Kcnq2"
                     /standard_name="long arm"
                     /function="long arm for homologous recombination"
                     /label=long arm
                     /note="Kcnq2 sequence stretching from intron 1 to intron 5,
                     which induce recombination at the homologous genomic region
                     in mouse ES cell"
     protein_bind    complement(7484..7517)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7518..8090
                     /gene="Kcnq2"
                     /note="Kcnq2 genomic segment consisting of exon 6 and
                     flanking intronic sequence, which can be exchanged with the
                     mutated segment via Cre-lox mediated specific 
                     recombination."
     protein_bind    complement(8093..8126)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(8183..8230)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        8301..8801
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             8815..9615
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     protein_bind    complement(9669..9702)
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(10041..10088)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    10145..11939
                     /gene="Kcnq2"
                     /standard_name="short arm"
                     /function="short arm for homologous recombination"
                     /label=short arm
                     /note="Kcnq2 sequence stretching from intron 6 to intron 8,
                     which induces recombination at the homologous genomic 
                     region in mouse ES cell"
     gene            10145..11939
                     /gene="Kcnq2"
                     /label=Kcnq2
     promoter        complement(11976..11994)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12015..12031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12039..12055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12063..12093)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12108..12129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12417..13005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13179..14036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(14037..14141)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gagcagtgtg gttttcaaga ggaagcaaaa agcctctcca cccaggcctg
      721 gaatgtttcc acccaatgtc gagcagtgtg gttttgcaag aggaagcaaa aagcctctcc
      781 acccaggcct ggaatgtttc cacccaatgt cgagcaaacc ccgcccagcg tcttgtcatt
      841 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt cccaggtcca cttcgcatat
      901 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc aggtcctcgc catggatcct
      961 gatgatgttg ttgattcttc taaatctttt gtgatggaaa acttttcttc gtaccacggg
     1021 actaaacctg gttatgtaga ttccattcaa aaaggtatac aaaagccaaa atctggtaca
     1081 caaggaaatt atgacgatga ttggaaaggg ttttatagta ccgacaataa atacgacgct
     1141 gcgggatact ctgtagataa tgaaaacccg ctctctggaa aagctggagg cgtggtcaaa
     1201 gtgacgtatc caggactgac gaaggttctc gcactaaaag tggataatgc cgaaactatt
     1261 aagaaagagt taggtttaag tctcactgaa ccgttgatgg agcaagtcgg aacggaagag
     1321 tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc tcagccttcc cttcgctgag
     1381 gggagttcta gcgttgaata tattaataac tgggaacagg cgaaagcgtt aagcgtagaa
     1441 cttgagatta attttgaaac ccgtggaaaa cgtggccaag atgcgatgta tgagtatatg
     1501 gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt gtgaaggaac cttacttctg
     1561 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     1621 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     1681 aacctatgga actgatgaat gggagcagtg gtggaatgca gatccactag agtcgaagcg
     1741 gcttcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     1801 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     1861 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     1921 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gaagggcaaa tcatttaaaa
     1981 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     2041 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     2101 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     2161 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     2221 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
     2281 cagcccgggg gatcctgatc cactagagcg gccgccgtgg cagcctggga aaggccagaa
     2341 agataatctg agttcttgat cagcaccccg atccagctgg aagagaattt ttctgcctct
     2401 gaacctagga aggaattgtt gcataccagg aaaaataatg aatatttacc ttctgtaggg
     2461 caggcagcgt ggagttggtt ctttttattg tttattgttt tttcactagc taaggctgcc
     2521 tggggtaaaa ccagacaagg atacagaaag gctggtcagc aatcagtgtg actgtggagt
     2581 cttgggtagc agaaaacagg gtgtggactg gccccctgct atgctctgag ggccctctaa
     2641 ggtctcaagt tctaggcatt ttagctaata ggcacgggct gcactgacca tggctggccc
     2701 actgactgcg tgtctctcac cttctgcagg ttccttttag tcttctcctg ccttgtgctt
     2761 tctgtgtttt ccaccatcaa ggagtacgag aagagctctg agggggccct ctacatcttg
     2821 gtaagcctca cacttgggca gggcatgggg agaggcctgg aggctgggaa atggacctct
     2881 gcagcagtct gcctcccaac cccggacctc tgcttctgct tctgccctca accctgatcg
     2941 tcggagtgag ccaggccttc tgcatacacg gtctcctcca gagtagttag tgttctttct
     3001 tgcacacaca gctactgttg ctcccagcat tgtggtgtag agacctcaag tcccttccca
     3061 gtccttctcc tagtccctag aagtgatata acaacaccca gcagttctca ccactccccc
     3121 ctccctttcc gccctgccct ctggagtctc cgtgctcagg tgcccccact gaagtatgtg
     3181 gtgctcccca gaagatgtgg gtgaaggggt gggaggccat gatgctcgct catataaacc
     3241 ctgtgacggg acaggggtgg gtgtcatgga aacgggactg taattcctct gttgccattg
     3301 gtaacggaga tggcccttca agaatgatcc cactagagtg cttttccacc cactttgccc
     3361 cactgccctg gagctgcctg aattctaaag gaacctgttc tccttttggt atggctggga
     3421 agtctctcag gtcagctctg ctgtgcccac ccctcacact gacactagag cctcccaatc
     3481 cagctcagaa gggcggtgag aaggggggtt cctctggtgg tctacaggcc aggacttgct
     3541 ggcagcagta agtccctgga gggtctgttt ctagcaggga ttcaatcctg gctgacatga
     3601 gctaggtgcc ctgtggggtt tgagctaagc ctgaaggtct gatttctagc atctgatccc
     3661 tggggcttta tccatccctg tcccgaaagt gtcatgtagt atagaggaag ccctcctaag
     3721 gcctgatagt gtcctcatcc gtgttcctcc cacctcccag gaaatcgtga ctatcgtggt
     3781 attcggtgtt gagtactttg tgaggatctg ggctgcaggc tgctgttgcc ggtatcgagg
     3841 ctggaggggc aggctcaagt ttgccaggaa gccgttctgt gtgattggtg aggcctggtg
     3901 gacatgccca ccagagttgg gtctggggag agagttgctt ctgtcttgtc cataatgggc
     3961 tgagtgagag ggtagggcct tctggaagtt ccttcttatc accgacttgg agttggaggt
     4021 gcaatggctg acagggccta ggtcttggga gggagacaca gtactgggca gtctctcctg
     4081 gacactacag gtaggcaggg tgggcactgt aaaccaccgt tcccagggcc ccacattacc
     4141 ttgcatgtct acggggccat tgagtcatgc tctgtggacc ccggggagga gatgctgtga
     4201 tctgtgcctg tctcccacag atatcatggt gctgattgcc tccattgctg tgctggctgc
     4261 tggttcccag ggcaatgtct ttgccacatc tgcgcttcgg agcttgcggt tcttgcaaat
     4321 cttgcggatg atccgtatgg accggagggg tggcacctgg aagctcttgg gatcggtagt
     4381 ctacgctcac agcaaggtga gcagcactga cccagcctgg agcctgctgt ttaccatgac
     4441 ttcaaggctc caggctgctc taagctgaag tagtcatggg ccaggcaaga tataaatgag
     4501 cctctccttt tatcccatgg ctgactcttc ctcacttgtc ctcagaagct attttctatt
     4561 cttagtgtga gcaatccttc ctggagctgg cagcccctgt agctgagcat ttcccttcct
     4621 ggttctgagg gcctggatcg atactctgcc tgaatcacct gcttagggtt tgtttaagct
     4681 gtggcttatg gtggctgcag accagggtac ttgtggggtg gctgtgggaa cacatttctg
     4741 tgttgaaaga gtcctcagtg gccaagacct gatttctgcc atgttgtcta tgggattttt
     4801 gtgctcttta tgttctcact gactcaaatg ctctctatcc tacctgtccc ccttttgtca
     4861 gccatggtct tgtgggggca ggcactgggc tctggggaag ttgacctccc tagaggtctg
     4921 gtctctccct gtctctgcac tgcacaccca gagtgttggg aactgagtat acgcctgttg
     4981 ggacctgtac cccttcttgg ggatatctcc acagtgctgg gaccccagct gctggctgtg
     5041 gggtatgtgt ttccccagcc atggacaggg tttttaaagg actgctgata ctcttctccc
     5101 aggtctgaaa tagtcagttg aaagttgcat tgtacagccg gcagtcactt cattatccag
     5161 gacaagctct gttgccatcc ctgaccagtg tctgctctaa gttgttcctt gctgccatcg
     5221 ggaggggatt ccatcctcca agtccccgag ctgcacagtt gagggcaggg ctcccatgct
     5281 gggcttccag gctgacctga gagagacaat gacgtggctt cctgcctttg caggagctgg
     5341 tgactgcctg gtacattggc ttcctctgcc tcatcctggc ctcatttctg gtgtacttgg
     5401 cagaaaaggg tgagaatgac cactttgaca cctacgcaga tgcactctgg tggggtctgg
     5461 taagtcctgg tcactggtca ccattccttt ctcctccaca tcctctggtt actgctatcc
     5521 taaggatata taccatgccc cttgggacct gaaggagaga cctagctgtc tttaggctgg
     5581 gctgggtggg aggacacctg gtggctggct gtggacataa tgacaactga ggctgatgtg
     5641 cttctagtgt tggtagatgt gacagactgg gttcctttct cccccttact acctgcaatt
     5701 ccccacttgg tgatggactg tgaaggagaa agattagggt gaaggaatat gggctctgag
     5761 agtaatgacc agaatagagc catcctaggg ctccccagct ccggagtttg aatgaccctc
     5821 ctaactttgg agcagtgtga gggtggaggt aacattgtta tcttggcctt ccattcccaa
     5881 gaccaactgc caccattcca ggtacttgct ggccttccgt ctccagtcaa tcctgccttt
     5941 gattgtgagg gacaagggag agttcaaagt gaagatggct cagtgggtaa agtacttgct
     6001 tcgaaagcat tgagatttca gtttggatct ccaggagccg tgtagaaatt aggtacatgt
     6061 gtgtgtgcac acacagacac acacagacac acagacatac acacacacac acagacacac
     6121 agacacacac acacacacac acacacacac acacacacac acacacagca aatggaaaac
     6181 acaggccctg actcactctc cgggatgttt tttaggtggt ctgcagcccg ctgacagtga
     6241 cttaattgaa gcttccccaa gacttgctgc tgcccaagtg gcttttgtgc ctgaggctcc
     6301 ccatcctgac tctgcctagg acactccaga agtcctttct cactgcccct ttccagaagg
     6361 gcctactggt tatctttgaa cttatgcttg aagtggacat ggaagacagg tttcctaggc
     6421 ccctgttttc tgatcttgct ttctggagcc cacactagcc tcgagaccta ctccaatgag
     6481 atagaaatgg gtttttccta gtccctcccc caatgcttta gactccttgg aggctcaggc
     6541 agaggctgtt gtcacttgac aagccacgag gtgatgacct aggcaggtgc tgtgcttgca
     6601 gagtcagggg ggagggttag acaactgccc ctagggtgaa gccacagggc agtttatttg
     6661 agatcttact aatgcccaca ggtctggaac cctgtgccct tttgggaaga agcagggcta
     6721 ttgggagtga cgggagttta agactggaga tgactggtga ggagattgtg tgattacagg
     6781 gcaggagcag ctggattgtc tgagtgtgta tgcccaggct ctgctgaggg aggaagcttg
     6841 gtaccctggg gtcctgaatt ctaaccagct ggccccaggg ttctgtgaga gcgatgtagc
     6901 ccattctttt cagaatgttc tcgtgggatg gggggggggc ggggagggta ggccttcctt
     6961 ctctttcaga actgaagcaa acattttatc tttattgttt caaagtaatc cttgtatttt
     7021 caaatgtcca tgggtttcta aaatgcagcc ccctcgctac tacccatctg tcttcttggc
     7081 taccctctac ccaccacccc ttacctcctc ccctcctggg cccaaccatc tcctagattg
     7141 tcccctattc tccctgaatc ctgctctcct aggcctttct gactcctaac actctgtgga
     7201 cctggaatgc ttgcacttcc ttgatcttgt cccctgactt ggtaggcacg tactggttaa
     7261 ctggtgcccc ttcctctgag cacagagcag gggctagaaa tgccagcaac tcatctggat
     7321 gaaaactctc cttgcttggt gccctatgca aacagtgtag tttatcctct catgtgtgaa
     7381 ttaggggagc cttgggaatg gttccccttt tagtgtagaa ttaagattgg ggattgtaga
     7441 ataaaggaaa gcctatgctg actctggcct tgtttggcac tagtaccgtt cgtatagcat
     7501 acattatacg aagttatcta gtatggatga tgaaggaagg atgacctcct gtaggtacag
     7561 ctgaagtctg aatgaccatc agtgtccagc ttcccctaaa ctacttggat gaacttccca
     7621 tggctgagca gggacatctg gcctgggatt gcgacctctg tggtaggcca tttccagcct
     7681 gcccaagtaa ccagagcccc ttacccctca gatcaccctg acgaccattg gctacgggga
     7741 caagtaccct cagacctgga acgggaggct gctggcagcg acctttaccc tcattggtgt
     7801 ctcgttcttt gctcttcctg ctgtgagtcc cgcgcacctt cctactctgg agatgttagg
     7861 ggttttagag gctccccata aggcttggcc atggcccacc tgtgaactgt gaacttggac
     7921 acagtcctgt ggggtttaca gaccctagag agctccagac tagaccagag tgaggctgct
     7981 ctggtttaga tgcctgcggc ctccctaggc acacttctag tgtcttcaga tcaggccttc
     8041 tcacatagct ctgttgattt ctgtggcttt cccatctgaa tgattctagc taataacttc
     8101 gtatagcata cattatacga agttatatta agggttccgg atccgatgat atcagatccc
     8161 catcaagctt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa
     8221 taggaacttc aagatccact agcgataagc tgcccccgac ctcgacgaat tcatcgatga
     8281 ttcgacctcg aaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     8341 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     8401 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     8461 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     8521 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     8581 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     8641 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     8701 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctcg cacgcttcaa aagcgcacgt
     8761 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggga
     8821 tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     8881 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     8941 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     9001 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     9061 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     9121 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     9181 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     9241 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     9301 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     9361 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     9421 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     9481 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     9541 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     9601 cttgacgagt tcttctgagg ggatctgata tcatcggatc ccccgggggc tgcaggtcga
     9661 gggacctaat aacttcgtat aggatacttt atacgaagtt atattaaggg ttccggatcc
     9721 tctagagtcg accccgggat gcagaattga tgatctatta acaataaaga tgtccactaa
     9781 aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt
     9841 gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa atgcctgctc
     9901 tttactgaag gctctttact attgctttat gataatgttt catagttgga tatcataatt
     9961 taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatcc
    10021 cccagcttca aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata
    10081 ggaacttcaa gatccactag cgataagctg cccccgacct cgacctgcag gcatgcaagc
    10141 tagatagtgg gggttctcat gtaccacata cagagtggga tggttatatt gactgctgca
    10201 ctggggctga cagagggcag ctgtctgtag ctgcttggtg gacagggcag gttaggaggg
    10261 ctcctgattg ggatcactag gcactgcctt ctgctaagca gaggttgcct gggcctggaa
    10321 gtgctagata attccaagga tgtaggaagg tgcacccaca aaacacaagt attgtaggca
    10381 agggagaaga gggccatggt cccaggatgt ggtcttacct tagtgacttg cagggcattt
    10441 tgggatccgg ctttgccctg aaagtccaag agcagcatcg gcaaaaacac tttgagaaac
    10501 ggcggaaccc tgcggcaggt ctgatccagg tgagccttag tccctgttag ggaccaaccc
    10561 ctgtcctgat ttcacctcac ccagaagtga acccagcagg atcctggcac tggcaatggc
    10621 tgtaccatgc ttttccctgt atatggatag gctgtagcct gtgtttggaa aaacaaagtt
    10681 gtgttcagca taagaaaaga ttatacatct gaggtgacac ctagtgactc atggtactgc
    10741 tgctggatgg acctattctc agtggtcact tggctcaggt catactgtct caggaaccat
    10801 ctaggttatt cccagtgtcc tgtgaggtaa gtgtctcatc acacctattc tgcagctgtg
    10861 agacccacag cttagagaag ttgagtctga tagcaagagg ctcctgaggc acatctgtga
    10921 cacttcggca tgagctgtgg acattgtgcc ttcctgcttt gcgcttgctc atgtggtctt
    10981 tccattcagg ggcaggtgag gtgtgcaggc cactgaacat gcatggagaa gatagagatt
    11041 cctcttgaac tctggagtta gccaggattt cctgccagat gaatgtggtc tctgtatctt
    11101 tctttttaaa aaaaaaaaaa aaaaaagatg tggccttcac tgtcctaacc ttgggtccaa
    11161 cttccttttg tgtctgtact gtatagcttt caccataaaa catatattgt tttgtaattt
    11221 caaaatatac aaaaaggaac agattgccca tttgaaaagt aaaataattg catgtccaag
    11281 gggccaaaag gtttaacagg gagcagcata aattgagtac cactcccata tatggaaaag
    11341 ccattcaaca gtaccctgtg agtctagtac caccaaagag caatagcata gctgttcctg
    11401 cctcatccac aagcccctcc ttatttgtct tgcctacagt ttccccactg tgaatttcag
    11461 ggtggttccc agagcttcac agcacctcca acccatttat gatgcatgac cacagggtga
    11521 aggtattgtg gtagaacaat tgatcaaaag gtcttccttt gaacaaagat gactttctac
    11581 atatttgggg gcaaagcttt ggttaacaat gactccagta gcccccatgt aggtacaggg
    11641 gtagaaaaac tgatttttca agaggattat cacctggtta tgattctggc actcaatcag
    11701 aaaaatactt ggaggcagag tctcagagga ggtaccaggc ctttcatgcc ttcaaagtgt
    11761 tctaaagaag tcagcctggc cggcctgtac cccatgtact ccttgatgtt attgaaagat
    11821 tgatggagac caaagtaagt aaaaagaaat cccatgttca gggactggat gaaataatct
    11881 cattcaaatg ttcttcccac acattggcat gcaggtccag tgccctccac ccccttcctg
    11941 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
    12001 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    12061 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    12121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    12181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    12241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    12301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    12361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    12421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    12481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    12541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    12601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    12661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    12721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    12781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    12841 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    12901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    12961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    13021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    13081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    13141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    13201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    13261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    13321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    13381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    13441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    13501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    13561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    13621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    13681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    13741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    13801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    13861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    13921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    13981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    14041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    14101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    14161 ccac
//
